| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human C-RAF around the phosphorylation site of Ser301. |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Phospho-C-RAF (Ser301). Phospho-C-RAF (Ser301) Antibody detects endogenous levels of C-RAF only when phosphorylated at Sersine 301. It is validated for Immunofluorescence, Immunohistochemistry, Western Blot.
Validated Applications
| Applications | Immunofluorescence, Immunohistochemistry, Western Blot |
|---|---|
| WB | 1:500-1:2000 |
| IF/ICC | 1:100-1:500 |
| IHC | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A synthesized peptide derived from human C-RAF around the phosphorylation site of Ser301.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | A synthesized peptide derived from human C-RAF around the phosphorylation site of Ser301. |
| Purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. |
| Formulation | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RRID | AB_2834093 |
| Storage | Store at -20 °C. Stable for 12 months from date of receipt. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. Confirm predicted species with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Phospho-C-RAF (Ser301) research from AFFINITY BIOSCIENCES LTD, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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