| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human STAT5 around the phosphorylation site of Ser725/Ser730. |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Phospho-STAT5 (Ser725/Ser730)[Ser726/Ser731]. Phospho-STAT5 (Ser725/Ser730) Antibody detects endogenous levels of STAT5 only when phosphorylated at Ser726/Ser731, which site historically referenced as Ser725/Ser730. It is validated for Immunofluorescence, Immunohistochemistry, Western Blot.
Validated Applications
| Applications | Immunofluorescence, Immunohistochemistry, Western Blot |
|---|---|
| WB | 1:500-1:2000 |
| IHC | 1:50-1:200 |
| IF/ICC | 1:100-1:500 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A synthesized peptide derived from human STAT5 around the phosphorylation site of Ser725/Ser730.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | A synthesized peptide derived from human STAT5 around the phosphorylation site of Ser725/Ser730. |
| Purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. |
| Formulation | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RRID | AB_2834723 |
| Storage | Store at -20 °C. Stable for 12 months from date of receipt. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. Confirm predicted species with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Phospho-STAT5 (Ser725/Ser730)[Ser726/Ser731] research from AFFINITY BIOSCIENCES LTD, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Discovery of Janus Kinase and Histone Deacetylase Dual Inhibitors as a New Strategy to Treat Psoriasis. Journal of medicinal chemistry (2024). [IF=6.8] PMID: 39415349
You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. Journal of Ethnopharmacology (2020). [IF=4.8] PMID: 31526861
Eldecalcitol effectively prevents alveolar bone loss by partially improving Th17/Treg cell balance in diabetes-associated periodontitis. Frontiers in bioengineering and biotechnology (2023). [IF=4.3] PMID: 36815882
Shengmaisan combined with Liuwei Dihuang Decoction alleviates chronic intermittent hypoxia-induced cognitive impairment by activating the EPO/EPOR/JAK2 signaling pathway. Chinese journal of natural medicines (2024). [IF=4.0] PMID: 38796216
Bleomycin triggers chronic mechanical nociception by activating TRPV1 and glial reaction-mediated neuroinflammation via TSLP/TSLPR/pSTAT5 signals. Brain research bulletin (2024). [IF=3.5] PMID: 39277019
Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus). European Journal of Histochemistry (2019). [IF=2.1] PMID: 30652434
Seasonal patterns of prolactin, prolactin receptor, and STAT5 expression in the ovaries of wild ground squirrels (Citellus dauricus Brandt). European Journal of Histochemistry (2023). [IF=2.1] PMID: 37781865
