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| Catalog no. (Mfr.) | |
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Product Overview
PolyJet™ DNA In Vitro Transfection Reagent rests on SignaGen's proprietary polymer synthesis technology and takes the form of a biodegradable, polymer-based DNA transfection reagent, giving effective and reproducible results in CHO, HeLa, NIH-3T3, COS-7, HEK293, and a wide span of mammalian cells that resist transfection. Even at very low concentration it halts DNA migration in electrophoresis, and the transfection complex is ready within 5 minutes at RT. What stands out is how the polymer breaks down, rapidly and completely, once the transfection complex has undergone endocytosis, leaving cytotoxicity much lower.
A 1.0 ml quantity of PolyJet™ reagent stretches to ~667 transfections in 24-well plates or ~333 in 6-well plates, which SignaGen positions as a very affordable alternative to the leading products where commonly used as well as hard-to-transfect mammalian cells must be transfected.
Specifications
| Transfection Type | DNA Transfection |
|---|---|
| Application Setting | In Vitro |
| Pre-Optimized | No — General-Purpose Formulation |
| Storage | Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |
Features
- Biodegradable once endocytosed
- Exceptionally high titers of virus production
- Works equally well with very long DNAs (>89 kb)
- Equally suited to single DNA transfection and multi DNA co-transfection
- High-level recombinant protein production
- Straightforward, robust transfection procedure
- Very low cost
Performance Data
Transfection Efficiency by Cell Line
| Cell Lines | Efficiency (% GFP) | Cell Lines | Efficiency (% GFP) |
|---|---|---|---|
| McArdle 7777 | 65-70% | hESCs | 70% |
| Hep3D | 67-76% | SN56 | 81% |
| SHEP | 68-71% | MC3T3-E1 | 80% |
| 3T3-442A | 35% | Primary melanocyte | 35% |
| COS-7 | 85-90% | mESCs | 70% |
| CV-1 | 60% | L929 | 59% |
| D 407 | 70% | MCF-7 | 68% |
| DHD Pro.b | 70% | MDCK | 68% |
| 3LL | 80% | Neuro2A | 86% |
| B16-F10 | 85% | NIH 3T3 | 76% |
| BAEC | 51% | PC12 | 50% |
| BHK-21 | 80% | SH-SY5Y | 25% |
| Ca Ski | 88% | SiHa | 60% |
| CaCo2 | 60% | SKOV3 | 65% |
| CHO | 88% | Huh-7 | 70% |
| HCS-2/8 | 61% | IGROV1 | 35% |
| HEK-293 | 86% | DF-1, Chicken Embryonic Cell | 50% |
| HeLa | 88% | 6CSFMEo | 71% |
| HLMEC | 72% | WEHI 231 | 26% |
| H-MVEC | 59% | A549 | 75% |
| Huh-7D12 | 72% | LNCap | 75 |
| ATT20 | 46% | Prim. mouse keratinocyte | 29% |
| SK-N-SH | 29% | Prim. human skin fibroblast | 50% |
| C2C12 | 46% | Prim. human pre-adipocyte | 32% |
| HepG2 | 72% | Prim. mouse embry. fibroblast | 30% |
Applications
This DNA transfection reagent supports a range of molecular biology applications including transient and stable transfection, reporter assays, protein expression studies, and gene function analysis in mammalian cell systems.
Safety & Handling
- General Protocol for Transfecting Mammalian Cells (PDF)
- Short Protocol – DNA Transfection (PDF)
- Protocol for Lentivirus Packaging in HEK293T Cells (PDF)
- Protocol for AAV Packaging in HEK293T Cells (PDF)
- Protocol for Adenovirus Packaging in HEK293A Cells (PDF)
- General Protocol for Transfecting HEK293 and CHO Cells in Suspension (PDF)
- Technical Note & Transfection Tips (PDF)
Can't Find What You're Looking For? We can help you source the best match or customize a transfection solution for your study. Options may include pre-optimized formulations for a cell line not listed here, matching transfection buffer supplied with the reagent, DNA, siRNA or in vivo formats within the same reagent family, and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Zhang C, He Y, Liu X, et al.. Oligomeric cystatin C supports the immunosuppressive activity of myeloid cells through interaction with inhibitory receptors. Signal Transduct Target Ther (2025) 10(1), 368.. 10.1038/s41392-025-02462-x
Cui X, Yang H, Cai C, et al.. Comparative characterization of human accelerated regions in neurons. Nature (2025) 640(8060), 991-999.. 10.1038/s41586-025-08622-x
Kimura K, Subramanian A, Yin Z, et al.. Immune checkpoint TIM-3 regulates microglia and Alzheimer's disease. Nature (2025) 641(8063), 718-731.. 10.1038/s41586-025-08852-z
Bezwada D, Perelli L, Lesner N, et al.. Mitochondrial complex I promotes kidney cancer metastasis. Nature (2024) 633(8031), 923-931.. 10.1038/s41586-024-07812-3
