| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant fragment of human Vimentin expressed in E. Coli. |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This monoclonal antibody (clone AFfirm079), raised in Mouse, recognizes Vimentin mouse monoclonal. Vimentin antibody detects endogenous levels of total Vimentin. It is validated for Western Blot, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:500-1:3000 |
| IHC | 1:50-1:200 |
| IF/ICC | 1:100-1:500 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Purified recombinant fragment of human Vimentin expressed in E. Coli.
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | AFfirm079 |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human Vimentin expressed in E. Coli. |
| Purification | Affinity-chromatography. |
| Formulation | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RRID | AB_2847777 |
| Storage | Store at -20 °C. Stable for 12 months from date of receipt. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. Confirm predicted species with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Vimentin mouse monoclonal research from AFFINITY BIOSCIENCES LTD, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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