Validated Cancer Antibodies

Application-validated cancer-research antibodies, each validated for two or more applications spanning Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications validated for your target. Save 15% with code ONCO15.

2679Products
2679 products
Image ProductProduct Type ApplicationPrice Status ACTION
Western blot analysis of FGF1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGF1 gene (2).
Target: FGF1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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Confocal immunofluorescent analysis of Hamartin antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Target: Hamartin
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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Rb antibody immunohsitochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma.
Target: Rb
Antibodies, Primary Antibodies Immunohistochemistry Western Blot Immunofluorescence ELISA
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Confocal immunofluorescent analysis of MMP7 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Target: MMP7
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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Immunofluorescent analysis of A549 cells using XRCC1 antibody at 1:25. Alexa Fluor 488-conjugated secondary was used (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 conjugated Phalloidin (red).
Target: XRCC1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Flow Cytometry Immunofluorescence ELISA
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Western blot analysis of HIF1 alpha antibody and CHO lysate. Routinely observed molecular weight: 100~120 kDa.
Target: HIF1 alpha
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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Western blot testing of phospho-ERBB4 antibody and FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min. Ab was used at 1:750. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Target: ERBB4
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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Fluorescent image of A549 cells stained with NPM1 antibody. Alexa Fluor 488 conjugated secondary was used (green). NPM1 immunoreactivity is localized to the nucleus and nucleolus significantly.
Target: NPM1
Antibodies, Primary Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence ELISA
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IHC analysis of FFPE human lymph tissue stained with NPM1 antibody
Target: NPM1
Antibodies, Primary Antibodies Immunohistochemistry Flow Cytometry Immunofluorescence Western Blot ELISA
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IHC analysis of FFPE human hepatocarcinoma tissue stained with PHB antibody
Target: PHB
Antibodies, Primary Antibodies Immunohistochemistry Western Blot Immunofluorescence Flow Cytometry ELISA
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Confocal immunofluorescent analysis of PML antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Target: PML
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry ELISA
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PGP9.5 antibody western blot analysis with (1) U266, (2) SH-SY5Y, (3) NCI-H1299, (4) mouse Neuro-2a, (5) rat C6, (6) PC-12 cell line and (7) mouse brain tissue lysate. Predicted molecular weight ~25 kDa.
Target: PGP9.5
Antibodies, Primary Antibodies Immunohistochemistry Flow Cytometry Immunofluorescence Western Blot ELISA
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PGP 9.5 antibody western blot analysis in (1) U266, (2) NCI-H1299, (3) mouse Neuro-2a cell line and (4) mouse brain tissue lysate. Predicted molecular weight ~25 kDa.
Target: PGP 9.5
Antibodies, Primary Antibodies Immunohistochemistry Immunofluorescence Western Blot ELISA
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Western blot testing of 1) mouse heart and 2) rat PC-12 lysate with SLUG antibody. Predicted molecular weight ~30 kDa.
Target: SLUG
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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Fluorescent confocal image of HeLa cells stained with SOX4 antibody. Alexa Fluor 488 conjugated secondary (green) was used. SOX4 immunoreactivity is localized to mitochondria strongly and nucleus weakly.
Target: SOX4
Antibodies, Primary Antibodies Immunofluorescence Western Blot Immunohistochemistry Flow Cytometry ELISA
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Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. Alexa Fluor 488 conjugated secondary (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LIN28B immunosignal is localized predominantly to the cytoplasm.
Target: LIN28B
Antibodies, Primary Antibodies Immunofluorescence Western Blot Immunohistochemistry Flow Cytometry ELISA
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Western blot analysis of lysate from 1) HL-60 and 2) Jurkat cell line using RUNX3 antibody at 1:1000. Predicted molecular weight: ~44 kDa, isoforms can be observed at 42-48 kDa.
Target: RUNX3
Antibodies, Primary Antibodies Immunohistochemistry Immunofluorescence Western Blot ELISA
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FOXO3 antibody western blot analysis in CEM lysate. Expected molecular weight: 71-90 kDa depending on glycosylation level.
Target: FOXO3
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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LDHA antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~36kDa.
Target: LDHA
Antibodies, Primary Antibodies Western Blot Immunofluorescence Immunohistochemistry ELISA
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Estrogen Receptor antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma.
Target: ESR1
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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PSMA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma.
Target: PSMA
Antibodies, Primary Antibodies Immunofluorescence Western Blot Immunohistochemistry ELISA
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Western blot analysis of lysate from 1) HepG2, 2) NCI-H460,3) Raji, and 4) Ramos cell line using MMP3 antibody at 1:1000. Predicted molecular weight ~54kDa.
Target: MMP3
Antibodies, Primary Antibodies Immunohistochemistry Immunofluorescence Western Blot ELISA
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PTEN antibody western blot analysis in K562 lysate.
Target: PTEN
Antibodies, Primary Antibodies Western Blot Immunofluorescence Immunohistochemistry ELISA
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Western blot analysis of CDKN2B antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the CDKN2B gene.
Target: CDKN2B
Antibodies, Primary Antibodies Immunofluorescence Flow Cytometry Western Blot Immunohistochemistry ELISA
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WT1 antibody western blot analysis in MCF-7 lysate. Predicted molecular weight: ~49 kDa
Target: WT1
Antibodies, Primary Antibodies Immunofluorescence Flow Cytometry Western Blot Immunohistochemistry ELISA
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MMP1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma
Target: MMP1
Antibodies, Primary Antibodies Immunohistochemistry Immunofluorescence Western Blot ELISA
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TGFBR2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue.
Target: TGFBR2
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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Confocal immunofluorescent analysis of TNFR antibody with U-251MG cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Target: TNFR
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry ELISA
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Confocal immunofluorescent analysis of JMJD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Target: JMJD3
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence ELISA
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CD44 antibody western blot analysis in HeLa lysate
Target: CD44
Antibodies, Primary Antibodies Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
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TGFB2 antibody immunohsitochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma
Target: TGFB2
Antibodies, Primary Antibodies Immunohistochemistry Immunofluorescence Western Blot ELISA
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Western blot analysis of lysate from K562 cell line using LTF antibody at 1:1000. Predicted molecular weight: ~78/73kDa (isoforms 1/2). (1)
Target: LTF
Antibodies, Primary Antibodies Immunohistochemistry Immunofluorescence Western Blot ELISA
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Western blot analysis of 293 lysate using SET07 antibody at 1:1000.
Target: SET07
Antibodies, Primary Antibodies Immunohistochemistry Immunofluorescence Western Blot ELISA
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Immunohistochemical analysis of paraffin-embedded human liver using MET antibody at 1:25 dilution.
Target: MET
Antibodies, Primary Antibodies Immunofluorescence Western Blot Immunohistochemistry ELISA
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Fluorescent confocal image of HepG2 cells stained with MET antibody. Note the highly specific localization of the MET immunosignal to the cytoplasm
Target: MET
Antibodies, Primary Antibodies Immunofluorescence Immunohistochemistry Western Blot ELISA
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Fluorescent confocal image of HepG2 cells stained with HGFR / MET antibody. Note the highly specific localization of the MET immunosignal to the cytoplasm.
Target: MET
Antibodies, Primary Antibodies Immunofluorescence Immunohistochemistry Western Blot ELISA
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About Validated Cancer Antibodies

Every antibody here is validated for multiple cancer-research applications — two or more of Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications and images validated for your specific target. Use code ONCO15 for 15% off cancer antibodies.

How validated is “validated”? Choosing a cancer antibody

Two antibodies to the same target are not interchangeable. The deciding factor is the evidence behind the listing — in which applications, species, and with what images. This collection sets the bar at multi-application validation: every antibody is validated in two or more of WB, IHC and IF.

Decision dimension Multi-application validated (this collection) Single-application Catalog / RUO-only
Validation evidenceTwo or more of WB / IHC / IF, with datasheet dataOne application (often WB only)Specification sheet; little or no application data
Applications confirmed2+ (across lysate, tissue and/or cells)10–1, frequently predicted
Reproducibility riskLower — cross-application concordanceModerate — format-dependentHigher — unverified
Best forPublication-grade tumor work across methodsA single, well-defined assayEarly screening on a budget
Watch-outsConfirm the validated applications, species and clone for your modelMay not transfer to IHC/IFPlan to validate in-house first

How to read this: if your project spans lysate, tissue and cells — or is headed for publication — start here, where every antibody is validated in two or more of these applications. Confirm on the datasheet which applications are validated for your target. If you only need one assay, the broader Cancer Antibodies catalog has more single-application options; for non-cancer targets browse all Antibodies. Validation is per-clone and per-lot — always check the datasheet image for your specific application and species.

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