BeaverBeads Magrose DEAE Anion Exchange Magnetic Beads

Product Overview

BeaverBeads™ Magrose DEAE are superparamagnetic agarose microspheres derivatised with diethylaminoethyl (DEAE) groups — the classical weak anion exchange ligand. Below their isoelectric point the DEAE groups are positively charged and bind negatively charged proteins, which are then released by raising ionic strength or shifting pH.

The magnetic format removes the hardware from ion exchange. There is no column to pack, no back-pressure to manage and no flow rate to control: beads are mixed with the sample, collected on a magnet, washed and eluted. That makes the technique practical for small volumes, for viscous or particulate samples that would foul a column, and for running many conditions in parallel during method development.

Selectivity and stability

DEAE is a weak anion exchanger, so its charge state varies with pH — useful when you want to tune selectivity by moving the working pH relative to the target's isoelectric point. The manufacturer reports that the beads retain a stable, high protein binding capacity even at pH 3 or pH 12, which gives room for aggressive cleaning and for capture conditions outside the usual range. Binding capacity itself depends on the charge distribution of the target protein; BEAVER publishes capacity figures as reference values only.

Applications

Anion exchange capture and polishing of recombinant and native proteins; glycoprotein purification, where the sialic acid content of the glycans provides negative charge; purification of nucleic acid-binding proteins; buffer-exchange-free capture from dilute samples; and rapid screening of ion exchange conditions during process development.

Related chemistries

Magrose DEAE sits in BEAVER's Magrose protein purification family alongside Heparin (cation exchange and heparin-binding proteins), GSH (GST-tagged proteins), IDA-Nickel and IDA-Cobalt (His-tagged proteins), Strep-Tactin (Strep-tag II) and Protein A, G and A/G (antibodies). All share the same 30–150 µm magnetic agarose matrix and the same handling.

Handling and safety

Do not freeze, dry out or centrifuge the beads — each causes irreversible aggregation. Resuspend fully before every use. A magnetic separator is required. Keep beads and separators away from metal objects, electronic devices and implanted electronic devices such as cardiac pacemakers.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. Specifications are those published by the manufacturer, BEAVER Biomedical Engineering Co., Ltd. A product-specific user manual for this catalogue number was not available at the time of listing; contact us if you need one before ordering.