Validated Cancer Antibodies

Application-validated cancer-research antibodies, each validated for two or more applications spanning Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications validated for your target. Save 15% with code ONCO15.

2679Products
2679 products
Image ProductProduct Type ApplicationPrice Status ACTION
CHEK1 Antibody – Figure 1 of 3
Target: CHEK1
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate
All lanes: RbAp48 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 48, 47, 44 kDa
Observed band size: 53, 40 kDa
Target: RBBP4
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A375 whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate
All lanes: ERK2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42, 37 kDa
Observed band size: 42 kDa
Target: MAPK1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: HAUSP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 129, 127 kDa
Observed band size: 140 kDa
Target: USP7
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate
All lanes: pro Caspase 3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Target: CASP3
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: U-251 whole cell lysate
All lanes: Mutant p53 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 53 kDa
Target: TP53
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
All lanes: CD47 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 32, 34 kDa
Observed band size: 55 kDa
Target: CD47
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
ALDOA Antibody – Figure 1 of 3
Target: ALDOA
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
AKT1 Antibody – Figure 1 of 3
Target: AKT1
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: Mad2L2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Target: MAD2L2
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, PC3 whole cell lysate, Mouse brain tissue, Rat brain tissue
All lanes: LOXL2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87 kDa
Observed band size: 53 kDa
Target: LOXL2
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
SIN3A Antibody – Figure 1 of 4
Target: SIN3A
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
PARP1 Antibody – Figure 1 of 5
Target: PARP1
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: COLO320 whole cell lysate, 293 whole cell lysate
All lanes: TP53 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 53 kDa
Target: TP53
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
KLF4 Antibody – Figure 1 of 5
Target: KLF4
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
E2F1 Antibody – Figure 1 of 5
Target: E2F1
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Exposure time: 120s
Target: EGFR
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: JK whole cell lysate(20µg) , K562 whole cell lysate(20µg) , SY5Y whole cell lysate(20µg) , HepG2 whole cell lysate(20µg) , COLO205 whole cell lysate(20µg) 
All lanes: CDK6 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 120s
Target: CDK6
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue
All lanes: PKM antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 59, 57 kDa
Observed band size: 58 kDa
Target: PKM
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate
All lanes: MET antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 156, 158, 86 kDa
Observed band size: 156 kDa
Target: MET
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in A549 whole cell lysate,HepG2 whole cell lysate(treated with EGF or not) 
All lanes Phospho-MAPK3 antibody at 2.35μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Target: MAPK3
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF) 
All lanes Phospho-RAF1 antibody at 1.18μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 KDa
Observed band size: 73 KDa
Target: RAF1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: CDKN1B antibody at 0.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23 KDa
Observed band size: 27 KDa
Target: CDKN1B
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Phospho-MTOR (S2448) Antibody – Figure 1 of 3
Target: MTOR
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not) 
All lanes Phospho-AKT1 antibody at 1.08μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 KDa
Observed band size: 60 KDa
Target: AKT1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate(treated with Calyculin A or not) 
All lanes Phospho-GSK3B antibody at 0.77μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 KDa
Observed band size: 46 KDa
Target: GSK3B
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain tissue, Mouse Brain tissue, Rabbit Skeletal Muscle tissue, Rat Kidney tissue, Rabbit Kidney tissue 
All lanes ENO1 antibody at 1:10000
Secondary
Goat polyclonal to mouse IgG at 0.261ug/ml
Predicted band size: 47 KDa
Observed band size: 47 KDa
Exposure time: 1min
Target: ENO1
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry Immunoprecipitation
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-PA005165LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Target: CEACAM5
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $166.00
Regular price From $166.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate
All lanes: PIK3R6 antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Target: PIK3R6
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $166.00
Regular price From $166.00
In Stock at Manufacturer
Western blot analysis of 1) Hela, 2)3T3, 3) Rat Brain Tissue with MEK2 Mouse mAb diluted at 1:2,000.
Target: MAP2K2
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Immunoprecipitation
From $119.00
Regular price From $119.00
In Stock at Manufacturer
TP53 Monoclonal Antibody – Figure 1 of 3
Target: TP53
Primary Antibody ELISA Western Blot Immunohistochemistry Immunofluorescence
From $119.00
Regular price From $119.00
In Stock at Manufacturer
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Target: EGFR
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
From $119.00
Regular price From $119.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Jurkat whole cell lysate, THP-1 whole cell lysate, Raji whole cell lysate
All lanes: CD31 antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 83, 81, 80, 82 kDa
Observed band size: 130 kDa
Target: CD31
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody.
Target: PTEN
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
$360.00
Regular price $360.00
In Stock at Manufacturer
Western blot analysis of extracts from 293 cells untreated or treated with HU using CDK6(phospho-Tyr13) Antibody.
Target: CDK6
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
$360.00
Regular price $360.00
In Stock at Manufacturer
Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3α/β(Phospho-Tyr279/216) Antibody.
Target: GSK3A/GSK3B
Antibodies, Primary Antibodies ELISA Western Blot Immunohistochemistry Immunofluorescence
$360.00
Regular price $360.00
In Stock at Manufacturer

About Validated Cancer Antibodies

Every antibody here is validated for multiple cancer-research applications — two or more of Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications and images validated for your specific target. Use code ONCO15 for 15% off cancer antibodies.

How validated is “validated”? Choosing a cancer antibody

Two antibodies to the same target are not interchangeable. The deciding factor is the evidence behind the listing — in which applications, species, and with what images. This collection sets the bar at multi-application validation: every antibody is validated in two or more of WB, IHC and IF.

Decision dimension Multi-application validated (this collection) Single-application Catalog / RUO-only
Validation evidenceTwo or more of WB / IHC / IF, with datasheet dataOne application (often WB only)Specification sheet; little or no application data
Applications confirmed2+ (across lysate, tissue and/or cells)10–1, frequently predicted
Reproducibility riskLower — cross-application concordanceModerate — format-dependentHigher — unverified
Best forPublication-grade tumor work across methodsA single, well-defined assayEarly screening on a budget
Watch-outsConfirm the validated applications, species and clone for your modelMay not transfer to IHC/IFPlan to validate in-house first

How to read this: if your project spans lysate, tissue and cells — or is headed for publication — start here, where every antibody is validated in two or more of these applications. Confirm on the datasheet which applications are validated for your target. If you only need one assay, the broader Cancer Antibodies catalog has more single-application options; for non-cancer targets browse all Antibodies. Validation is per-clone and per-lot — always check the datasheet image for your specific application and species.

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